http://vivian2008.blogbus.com/Mon, 09 Nov 2009 11:49:01 GMT静静行走。A little somewhere I go,a little something I do,reminds me of you.博客大巴http://vivian2008.blogbus.com/atom.xmlMon, 09 Nov 2009 11:49:01 GMThttp://public.blogbus.com/profile/8/6/2/1145268/avatar_1145268_96.jpghttp://vivian2008.blogbus.com/http://vivian2008.blogbus.com/logs/50788871.htmlvivian*http://vivian2008.blogbus.com/logs/50788871.html<p>所有的所有的快乐都来吧，让我开心地拥抱你用残存的热情</p><!--sp--><br /><br /><div class="sysmsg"><b><a href="http://www.blogbus.com" target="_blank">博客大巴，你的个人传媒早班车</a></b></div><br /><br /><div class="addfav">收藏到：<span class= "delicious"><a href="http://delicious.com/save?url=http%3A%2F%2Fvivian2008.blogbus.com%2Flogs%2F50788871.html&title=%E5%8E%BB%E4%BA%86%E9%9B%8D%E5%92%8C%E5%AE%AB">Del.icio.us</a></span></div><img src="http://www1.feedsky.com/t1/294142758/vivian2008_blogbus_com/blogbus.com/s.gif?r=http://vivian2008.blogbus.com/logs/50788871.html" border="0" height="0" width="0" style="position:absolute" /><p class="fswww1"><a href="http://www1.feedsky.com/r/l/blogbus.com/vivian2008_blogbus_com/294142758/art01.html" target="_blank"><img border="0" ismap="ismap" src="http://www1.feedsky.com/r/i/blogbus.com/vivian2008_blogbus_com/294142758/art01.gif" onerror="this.style.display='none'" /></a></p>未分类Sat, 07 Nov 2009 19:44:35 +0800http://vivian2008.blogbus.com/logs/50788871.htmlvivian*http://vivian2008.blogbus.com/logs/50788871.htmlhttp://vivian2008.blogbus.com/atom.xmlblogbus.com/vivian2008_blogbus_com/~6904537/294142758/5012461http://vivian2008.blogbus.com/logs/49777628.htmlvivian*http://vivian2008.blogbus.com/logs/49777628.html<p>这世界上有无缘无故的爱么？有无缘无故的恨么？。。。请不要恨我，更不要爱我。</p> <p>不喜欢被人牵着鼻子走的感觉。</p> <p>更不喜欢一点小事算计来算计去的还装得自己很牛叉。</p> <p>看朋友在QQ签名骂人觉得很痛快，自己想骂人却从来骂不出口。做人做的这样猥琐，真是打心眼里瞧不起自己。</p> <p>甲流甲流你快些走。。God Bless Beijing,God Bless China.</p><!--sp--><br /><br /><div class="sysmsg"><b><a href="http://www.blogbus.com" target="_blank">博客大巴，你的个人传媒早班车</a></b></div><br /><br /><div class="addfav">收藏到：<span 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+0800http://vivian2008.blogbus.com/logs/49777628.htmlvivian*http://vivian2008.blogbus.com/logs/49777628.htmlhttp://vivian2008.blogbus.com/atom.xmlblogbus.com/vivian2008_blogbus_com/~6904537/294142759/5012461http://vivian2008.blogbus.com/logs/49140867.htmlvivian*http://vivian2008.blogbus.com/logs/49140867.htmlhttp://hi.baidu.com/%B1%B1%BE%A9%CB%F7%C0%B3%B1%A6%B9%AB%CB%BE/blog/item/1662b3d808517ae039012f9f.html<br /><br /> 胶原酶Ⅰ<br />产品编号&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 规 格&nbs...<!--sp--><br /><br /><div class="sysmsg"><b><a href="http://www.blogbus.com" target="_blank">博客大巴，你的个人传媒早班车</a></b></div><br /><br /><div class="addfav">收藏到：<span class= "delicious"><a href="http://delicious.com/save?url=http%3A%2F%2Fvivian2008.blogbus.com%2Flogs%2F49140867.html&title=%E5%87%A0%E7%A7%8D%E8%83%B6%E5%8E%9F%E9%85%B6%E7%9A%84%E5%8C%BA%E5%88%AB">Del.icio.us</a></span></div><img src="http://www1.feedsky.com/t1/294142760/vivian2008_blogbus_com/blogbus.com/s.gif?r=http://vivian2008.blogbus.com/logs/49140867.html" border="0" height="0" width="0" style="position:absolute" /><p class="fswww1"><a href="http://www1.feedsky.com/r/l/blogbus.com/vivian2008_blogbus_com/294142760/art01.html" target="_blank"><img border="0" ismap="ismap" src="http://www1.feedsky.com/r/i/blogbus.com/vivian2008_blogbus_com/294142760/art01.gif" onerror="this.style.display='none'" /></a></p>未分类Sun, 25 Oct 2009 19:19:49 +0800http://vivian2008.blogbus.com/logs/49140867.htmlvivian*http://vivian2008.blogbus.com/logs/49140867.htmlhttp://vivian2008.blogbus.com/atom.xmlblogbus.com/vivian2008_blogbus_com/~6904537/294142760/5012461http://vivian2008.blogbus.com/logs/49137143.htmlvivian*http://vivian2008.blogbus.com/logs/49137143.html<p>http://www.flowcyto.cn/question.php?qid=217<br /><br />倪万茂译自华盛顿大学STEWART实验室相关资料<br /><br />第1天<br /><br />用100&mu;M的BrdU工作液（用5mg/ml的储存液以6&mu;L/mL稀释而成）标记细胞。<br />&Oslash;胚胎干细胞，30分钟<br />&Oslash;3T3细胞，2.5小时<br />&Oslash;二倍体成纤维细胞，4－6小时 胰酶消化细胞，...</p><!--sp--><br /><br /><div class="sysmsg"><b><a href="http://www.blogbus.com" target="_blank">博客大巴，你的个人传媒早班车</a></b></div><br /><br /><div class="addfav">收藏到：<span class= "delicious"><a href="http://delicious.com/save?url=http%3A%2F%2Fvivian2008.blogbus.com%2Flogs%2F49137143.html&title=BrdU%E6%8E%BA%E5%85%A5%E8%AF%95%E9%AA%8C%E8%AF%A6%E7%BB%86%E6%AD%A5%E9%AA%A4">Del.icio.us</a></span></div><img src="http://www1.feedsky.com/t1/294142761/vivian2008_blogbus_com/blogbus.com/s.gif?r=http://vivian2008.blogbus.com/logs/49137143.html" border="0" height="0" width="0" style="position:absolute" /><p class="fswww1"><a href="http://www1.feedsky.com/r/l/blogbus.com/vivian2008_blogbus_com/294142761/art01.html" target="_blank"><img border="0" ismap="ismap" src="http://www1.feedsky.com/r/i/blogbus.com/vivian2008_blogbus_com/294142761/art01.gif" onerror="this.style.display='none'" /></a></p>未分类Sun, 25 Oct 2009 18:34:42 +0800http://vivian2008.blogbus.com/logs/49137143.htmlvivian*http://vivian2008.blogbus.com/logs/49137143.htmlhttp://vivian2008.blogbus.com/atom.xmlblogbus.com/vivian2008_blogbus_com/~6904537/294142761/5012461http://vivian2008.blogbus.com/logs/48926552.htmlvivian*http://vivian2008.blogbus.com/logs/48926552.html<p>10%中性甲醛液<br />浓甲醛&nbsp;10ml<br />蒸馏水&nbsp;90ml<br />碳酸钙&nbsp;加至饱和</p><!--sp--><br /><br /><div class="sysmsg"><b><a href="http://www.blogbus.com" target="_blank">博客大巴，你的个人传媒早班车</a></b></div><br /><br /><div class="addfav">收藏到：<span class= "delicious"><a href="http://delicious.com/save?url=http%3A%2F%2Fvivian2008.blogbus.com%2Flogs%2F48926552.html&title=%E7%97%85%E7%90%86%E5%88%B6%E7%89%87%E6%8A%80%E6%9C%AF%E7%9A%84%E8%A7%84%E8%8C%83%EF%BC%88%E4%B8%80%EF%BC%89">Del.icio.us</a></span></div><img src="http://www1.feedsky.com/t1/294142762/vivian2008_blogbus_com/blogbus.com/s.gif?r=http://vivian2008.blogbus.com/logs/48926552.html" border="0" height="0" width="0" style="position:absolute" /><p class="fswww1"><a href="http://www1.feedsky.com/r/l/blogbus.com/vivian2008_blogbus_com/294142762/art01.html" target="_blank"><img border="0" ismap="ismap" src="http://www1.feedsky.com/r/i/blogbus.com/vivian2008_blogbus_com/294142762/art01.gif" onerror="this.style.display='none'" /></a></p>未分类Thu, 22 Oct 2009 12:27:20 +0800http://vivian2008.blogbus.com/logs/48926552.htmlvivian*http://vivian2008.blogbus.com/logs/48926552.htmlhttp://vivian2008.blogbus.com/atom.xmlblogbus.com/vivian2008_blogbus_com/~6904537/294142762/5012461http://vivian2008.blogbus.com/logs/48538338.htmlvivian*http://vivian2008.blogbus.com/logs/48538338.html做Real Time PCR的大体有四个目的。<br /><br /> 1. 检测某个基因表达水平的上调或下调。<br /><br /> 2. 检测siRNA靶基因的下调量。<br /><br /> 3. 观测某个基因在一系列细胞中的表达模式。<br /><br /> 4. 别出心裁的实验设计。<br /><br /> 四种实验对应的数据处理方法是不同的。但原理是一样的，即都基于这个公式：<br /><br /> Ct&nbsp;&nbsp; =&nbsp;&nbsp; -k * log...<!--sp--><br /><br /><div class="sysmsg"><b><a href="http://www.blogbus.com" target="_blank">博客大巴，你的个人传媒早班车</a></b></div><br /><br /><div class="addfav">收藏到：<span class= "delicious"><a href="http://delicious.com/save?url=http%3A%2F%2Fvivian2008.blogbus.com%2Flogs%2F48538338.html&title=Real+Time+PCR%E7%BB%93%E6%9E%9C%E5%88%86%E6%9E%90%E4%B8%8E%E5%AE%9E%E9%AA%8C%E8%AE%BE%E8%AE%A1">Del.icio.us</a></span></div><img src="http://www1.feedsky.com/t1/294142763/vivian2008_blogbus_com/blogbus.com/s.gif?r=http://vivian2008.blogbus.com/logs/48538338.html" border="0" height="0" width="0" style="position:absolute" /><p class="fswww1"><a href="http://www1.feedsky.com/r/l/blogbus.com/vivian2008_blogbus_com/294142763/art01.html" target="_blank"><img border="0" ismap="ismap" src="http://www1.feedsky.com/r/i/blogbus.com/vivian2008_blogbus_com/294142763/art01.gif" onerror="this.style.display='none'" /></a></p>未分类Fri, 16 Oct 2009 20:19:50 +0800http://vivian2008.blogbus.com/logs/48538338.htmlvivian*http://vivian2008.blogbus.com/logs/48538338.htmlhttp://vivian2008.blogbus.com/atom.xmlblogbus.com/vivian2008_blogbus_com/~6904537/294142763/5012461http://vivian2008.blogbus.com/logs/46681659.htmlvivian*http://vivian2008.blogbus.com/logs/46681659.html<p>Confocal 显微镜居然坏了！！2天的心血啊。。。听LF的建议做好的爬片放到了-20℃，但愿荧光不要衰减啊，虽然加了防淬灭剂，心里还是担心又担心。</p> <p>放在细胞间里的加样枪没了，郁闷。把东西整理了一下，放到外面来了。</p><!--sp--><br /><br /><div class="sysmsg"><b><a href="http://www.blogbus.com" target="_blank">博客大巴，你的个人传媒早班车</a></b></div><br /><br /><div class="addfav">收藏到：<span class= "delicious"><a href="http://delicious.com/save?url=http%3A%2F%2Fvivian2008.blogbus.com%2Flogs%2F46681659.html&title=%E6%BF%80%E5%85%89%E5%85%B1%E8%81%9A%E7%84%A6%E6%A0%B7%E5%93%81%E7%9A%84%E5%88%B6%E5%A4%87">Del.icio.us</a></span></div><img src="http://www1.feedsky.com/t1/294142764/vivian2008_blogbus_com/blogbus.com/s.gif?r=http://vivian2008.blogbus.com/logs/46681659.html" border="0" height="0" width="0" style="position:absolute" /><p class="fswww1"><a href="http://www1.feedsky.com/r/l/blogbus.com/vivian2008_blogbus_com/294142764/art01.html" target="_blank"><img border="0" ismap="ismap" src="http://www1.feedsky.com/r/i/blogbus.com/vivian2008_blogbus_com/294142764/art01.gif" onerror="this.style.display='none'" /></a></p>未分类Thu, 17 Sep 2009 20:20:16 +0800http://vivian2008.blogbus.com/logs/46681659.htmlvivian*http://vivian2008.blogbus.com/logs/46681659.htmlhttp://vivian2008.blogbus.com/atom.xmlblogbus.com/vivian2008_blogbus_com/~6904537/294142764/5012461http://vivian2008.blogbus.com/logs/44992397.htmlvivian*http://vivian2008.blogbus.com/logs/44992397.html<p>玫瑰小花的裙子好漂亮啊。。。喜欢。。下雨也要穿两天。</p><!--sp--><br /><br /><div class="sysmsg"><b><a href="http://www.blogbus.com" target="_blank">博客大巴，你的个人传媒早班车</a></b></div><br /><br /><div class="addfav">收藏到：<span class= "delicious"><a href="http://delicious.com/save?url=http%3A%2F%2Fvivian2008.blogbus.com%2Flogs%2F44992397.html&title=%E5%8F%88%E6%90%AC%E5%AE%B6%E4%BA%86">Del.icio.us</a></span></div><img src="http://www1.feedsky.com/t1/294142765/vivian2008_blogbus_com/blogbus.com/s.gif?r=http://vivian2008.blogbus.com/logs/44992397.html" border="0" height="0" width="0" style="position:absolute" /><p class="fswww1"><a href="http://www1.feedsky.com/r/l/blogbus.com/vivian2008_blogbus_com/294142765/art01.html" target="_blank"><img border="0" ismap="ismap" src="http://www1.feedsky.com/r/i/blogbus.com/vivian2008_blogbus_com/294142765/art01.gif" onerror="this.style.display='none'" /></a></p>未分类Mon, 24 Aug 2009 20:22:46 +0800http://vivian2008.blogbus.com/logs/44992397.htmlvivian*http://vivian2008.blogbus.com/logs/44992397.htmlhttp://vivian2008.blogbus.com/atom.xmlblogbus.com/vivian2008_blogbus_com/~6904537/294142765/5012461http://vivian2008.blogbus.com/logs/44153556.htmlvivian*http://vivian2008.blogbus.com/logs/44153556.html<p>知止而后能定。</p><!--sp--><br /><br /><div class="sysmsg"><b><a href="http://www.blogbus.com" target="_blank">博客大巴，你的个人传媒早班车</a></b></div><br /><br /><div class="addfav">收藏到：<span class= "delicious"><a href="http://delicious.com/save?url=http%3A%2F%2Fvivian2008.blogbus.com%2Flogs%2F44153556.html&title=%E5%86%92%E4%B8%AA%E6%B3%A1%E5%84%BF">Del.icio.us</a></span></div><img src="http://www1.feedsky.com/t1/294142766/vivian2008_blogbus_com/blogbus.com/s.gif?r=http://vivian2008.blogbus.com/logs/44153556.html" border="0" height="0" width="0" style="position:absolute" /><p class="fswww1"><a href="http://www1.feedsky.com/r/l/blogbus.com/vivian2008_blogbus_com/294142766/art01.html" target="_blank"><img border="0" ismap="ismap" src="http://www1.feedsky.com/r/i/blogbus.com/vivian2008_blogbus_com/294142766/art01.gif" onerror="this.style.display='none'" /></a></p>未分类Fri, 14 Aug 2009 09:01:06 +0800http://vivian2008.blogbus.com/logs/44153556.htmlvivian*http://vivian2008.blogbus.com/logs/44153556.htmlhttp://vivian2008.blogbus.com/atom.xmlblogbus.com/vivian2008_blogbus_com/~6904537/294142766/5012461http://vivian2008.blogbus.com/logs/41535241.htmlvivian*http://vivian2008.blogbus.com/logs/41535241.html<p>光看试剂盒说明书是远远不够的。</p> <p>忙碌度五颗星的一天。超负荷。小S同学又闯祸了。还好JY帮关了电磁炉，否则后果不堪设想。。。</p> <p>只好明天自己重压琼脂糖了。但愿细胞挺得过去。</p> <p>师姐们的预答辩，可惜一直困在细胞间呀。周日是正式的。</p><!--sp--><br /><br /><div class="sysmsg"><b><a href="http://www.blogbus.com" target="_blank">博客大巴，你的个人传媒早班车</a></b></div><br /><br /><div class="addfav">收藏到：<span class= "delicious"><a 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